Part:BBa_K2302009:Design

His＋Lpp-ompA＋L-FABP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 462
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 687
  

Design Notes

the plasmid is transformed into E. coli (DE3) for protein expression.

Source

Lpp-OmpA and FABP are chemically synthesized, and subcloned into the expression plasmid pET-14b.